CD40 Ligand Protein (CD40LG) (AA 108-261, N-Term)

N° du produit ABIN2666789

Cette protéine Recombinant CD40 Ligand est exprimée dans HEK-293 Cells.

Aperçu rapide pour CD40 Ligand Protein (CD40LG) (AA 108-261, N-Term) (ABIN2666789)

Antigène: CD40 Ligand (CD40LG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: Flow Cytometry (FACS)

Pureté: > 95 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 108-261, N-Term

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: ED50 = 20-100 ng/ml, corresponding to a specific activity of 1-5 x104 units/mg, as determined by the dose dependent stimulation of human B cell proliferation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40L

Sujet: CD40L is a type II membrane protein that shares 78 % amino acid identity with its mouse counterpart. Human CD40L is a polypeptide of 261 amino acids, consisting of 22, 24, and 215 amino acids for the cytoplasmic, transmembrane, and extracellular domains respectively. Soluble forms of CD40L (sCD40L) have been identified, and a 18 kD fragment has the ability to form homotrimers and bind to CD40 to induce biological signals. The 18 kD protein has full activity in B cell proliferation and differentiation assays. Activated platelets express CD40L on their surface and constitute the major source of sCD40L (>95 % in blood). Platelet-derived CD40L regulates neutrophil recruitment and lung damage in sepsis, and it has been suggested that MMP9 regulates CD40L shedding in abdominal sepsis. Platelet-associated CD40L has been linked to progression of atherosclerotic disease, and elevated plasma levels of sCD40L in patients seems to be a reliable predictor of cardiovascular events, such as atherosclerotic plaque rupture and acute coronary syndromes. Also, platelet-associated CD40L expression is increased in idiopathic thrombocytopenic purpura patients, and it has been speculated that platelet-associated-CD40L drives the activation of autoreactive B lymphocytes in this disease. In addition, high plasma levels of sCD40L have been connected to HIV-Associated Neurocognitive Disorders (HAND).

Poids moléculaire: The 175 amino acid recombinant protein has a predicted molecular mass of approximately 19 kDa. The DTT-reduced and non-reduced protein migrate at approximately 25 kDa by SDS-PAGE. The N-terminal amino acid contains 9His-2(SGGG)-IEGR-tag.

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints